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Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR▿

机译：TaqMan PCR实时定量评估奶酪样品中四环素抗性基因库

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摘要

A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.

机译：开发了TaqMan实时PCR分析法以定量零售奶酪中存在的tetS基因库。该协议为评估富含脂肪和蛋白质的食物样品中的抗生素抗性基因提供了一种快速，特异性，灵敏且与培养无关的方法。

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Manuzon, Michele Y.; Hanna, Scott E.; Luo, Hongliang; Yu, Zhongtang; Harper, W. James; Wang, Hua H.;
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年度 2007
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正文语种 en
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专利

1. Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR [J] . Michele Y. Manuzon, Scott E. Hanna, Hongliang Luo, Applied and Environmental Microbiology . 2007,第5期

机译：实时TaqMan PCR定量评估奶酪样品中四环素抗性基因库
2. Development of a versatile TaqMan? real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA [J] . Vijay J Gadkar, Martin Filion BMC Biotechnology . 2013,第1期

机译：开发多功能TaqMan？实时定量PCR（RT-qPCR）兼容锚序列，用于定量分析含有残留基因组DNA的RNA样品中的细菌基因转录本
3. Quantitative Assessment of Human β-Globin Gene Expression In Vitro by TaqMan Real-Time Reverse Transcription-PCR: Comparison with Competitive Reverse Transcription-PCR and Application to Mutations or Deletions in Noncoding Regions [J] . Annie Robert, Jean-Luc Gala, Leonid M. Irenge Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2005,第12期

机译：TaqMan实时逆转录PCR定量评估人β球蛋白基因的体外表达：与竞争性逆转录PCR的比较及其在非编码区的突变或缺失的应用
4. Validation of a Real-Time Quantitative PCR Method for Quantitation of Vector DNA in Mouse Tissue Samples Following Vector-Mediated Gene Delivery of HIV Antibody [C] . Angela Keightley, Marie-Soleil Christin-Piche, Jim Ackl, Molecular Medicine TRI-CON. . 2014

机译：在载体介导的HIV抗体递送后小鼠组织样品中载体DNA的实时定量PCR方法的实时定量PCR方法
5. Culturable bacterial endophytes from bermudagrass and TaqMan RTM real-time PCR to quantify Ophiosphaerella herpotricha. [D] . Hironaka, Janice Lynn. 2005

机译：来自百慕大草和TaqMan RTM实时PCR的可培养细菌内生菌，可定量鉴定蛇蝎Ophiosphaerella herpotricha。
6. Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR [O] . Michele Y. Manuzon, Scott E. Hanna, Hongliang Luo, 2007

机译：实时TaqMan PCR定量评估奶酪样品中四环素抗性基因库
7. Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA [O] . Vijay J Gadkar, Martin Filion 2013

机译：开发通用的TaqMan™实时定量PCR（RT-qPCR）兼容锚序列，以定量分析含有残留基因组DNA的RNA样品中的细菌基因转录本
8. Real-Time pcr Assay for Detection of Tetracycline Resistance Genes of Gram-Negative Bacteria; Conference paper [R] . Fan, W., Hartman, A. B., Lindler, L. 2003

机译：用于检测革兰氏阴性细菌四环素抗性基因的实时pcr检测;会议文件

Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR▿

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